ELISA of mouse immunoglobulins shows RMG06 reacts to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG06 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RMG06 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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